Figure 5

Functional traits of the increasing bacterial indicator taxa (detected in ETV0 and GUN0, in red) compared to control forest soil bacteria (detected in ETV15 and GUN15, in green) based on Bactotraits (A) and Tax4Fun (B) analyses. Only traits with significant differences between the 2 populations were represented (except for few functional pathways at the Level2 on plot B) and labelled with a * meaning that Wilcoxon rank sum tests had a p value < 0.05. Trait attributes are expressed as mean percentage (n = 6 for increasing Indicator taxa, and n = 5 for forest bacterial community) and ± standard deviation. (A) Morphological (Gram staining: the rest are Gram negatives, and motility: the rest are motile bacteria possessing flagella) and physiological (trophic type as lithotroph, i.e. bacteria that obtains its energy (electron donor) from inorganic compounds (iron, sulfur…) in opposition to organotroph, and oxygen preference: all attribute classes are shown) and affiliation to functional groups (5 functional groups were defined, no difference was found for stress-tolerant group) were assessed using BactoTraits (Cébron et al. 2021). (B) Genomic traits, i.e. KEGG functional pathways shown at Level 1, Level 2 and Level 3. Only functions representing more than 0.1% were represented. M: Metabolism, GIP: Genetic information processing, EIP: Environmental Information Processing and CP: Cellular Processes.