Table 2 Microbial mineralization activities, abundances (16S and 18S rRNA gene copy numbers for bacteria and fungi, respectively) and alpha-diversity indices (Chao1 richness, Shannon diversity and Pielou’s evenness) along longitudinal and depth gradients.

From: Taxonomic and functional trait-based approaches suggest that aerobic and anaerobic soil microorganisms allow the natural attenuation of oil from natural seeps

 

Sampling date

Distance from oil seep (m)

Aerobic mineralization CO2 production (µg C-CO2 g−1 d−1)

Anaerobic mineralization CO2 production (µg C-CO2 g−1 d−1)

Bacteria

Fungi

16S rRNA gene copies (× 1010 g−1)

Chao 1 richness

Shannon diversity H’

Pielou’s evenness

18S rRNA gene copies (× 108 g−1)

Chao 1 richness

Shannon diversity H’

Pielou’s evenness

GUN0

May 2016

0

248 ± 90 a

61 ± 5 a

8.7 ± 3.3 ab

6532 ± 441

6.0 ± 0.4 b

0.70 ± 0.04 b

10.0 ± 1.6

349 ± 10

3.05 ± 0.16 b

0.55 ± 0.03 b

GUN2

2

94 ± 7 b

31 ± 1 b

9.6 ± 2.2 ab

7292 ± 676

7.3 ± 0.2 a

0.83 ± 0.02 a

12.8 ± 4.6

429 ± 56

3.72 ± 0.22 a

0.64 ± 0.02 a

GUN4

4

76 ± 13 b

26 ± 5 b

10.0 ± 2.1 a

7988 ± 435

7.2 ± 0.4 a

0.82 ± 0.04 a

26.7 ± 16.1

448 ± 79

3.29 ± 0.39 ab

0.57 ± 0.05 ab

GUN10

10

61 ± 25 b

26 ± 9 b

5.1 ± 0.3 b

7156 ± 573

7.2 ± 0.2 a

0.82 ± 0.02 a

14.7 ± 3.9

426 ± 6

2.55 ± 0.18 c

0.45 ± 0.03 c

GUN15

15

47 ± 11 b

18 ± 4 b

6.0 ± 1.6 ab

7697 ± 158

7.3 ± 0.0 a

0.84 ± 0.01 a

14.4 ± 6.7

460 ± 65

3.45 ± 0.14 ab

0.59 ± 0.02 ab

ETV0

May 2016

0

192 ± 39 a

32 ± 12 a

6.9 ± 1.7

4683 ± 2150 b

5.1 ± 1.3 b

0.62 ± 0.13 b

8.2 ± 3.8

450 ± 44

3.28 ± 0.38

0.56 ± 0.06

ETV2

2

134 ± 72 ab

38 ± 7 a

8.4 ± 1.7

8630 ± 711 a

7.5 ± 0.1 a

0.84 ± 0.02 a

48.1 ± 61.3

421 ± 154

2.79 ± 1.87

0.47 ± 0.30

ETV4

4

56 ± 46 b

18 ± 8 ab

8.5 ± 3.0

7908 ± 944 a

7.3 ± 0.3 a

0.83 ± 0.03 a

12.2 ± 0.1

498 ± 69

3.59 ± 0.55

0.60 ± 0.08

ETV10

10

44 ± 9 b

13 ± 7 b

5.6 ± 1.0

8670 ± 882 a

7.5 ± 0.3 a

0.84 ± 0.02 a

7.9 ± 2.3

562 ± 57

3.78 ± 0.06

0.62 ± 0.00

ETV15

15

42 ± 7 b

7 ± 5 b

6.1 ± 0.7

7198 ± 1361 a

7.1 ± 0.2 a

0.81 ± 0.01 a

11.4 ± 1.4

492 ± 56

3.50 ± 0.21

0.59 ± 0.02

ETV0_P0-2

May 2017

0

207 ± 29 a

31 ± 3 a

10.6 ± 2.0 a

3539 ± 732 b

4.2 ± 0.6 c

0.53 ± 0.06 c

6.5 ± 1.9

355 ± 5

3.03 ± 0.23

0.54 ± 0.05

ETV0_P2-5

0

88 ± 16 b

24 ± 6 ab

6.1 ± 0.9 ab

4988 ± 479 a

5.1 ± 0.3 b

0.61 ± 0.03 b

3.0 ± 0.5

395 ± 35

2.88 ± 0.62

0.50 ± 0.10

ETV0_P5-15

0

51 ± 35 bc

16 ± 8 b

5.1 ± 2.2 b

5204 ± 921 a

6.4 ± 0.4 a

0.76 ± 0.04 a

5.2 ± 1.6

303 ± 56

2.15 ± 0.70

0.40 ± 0.11

ETV0_P15-30

0

15 ± 5 c

4 ± 1 c

2.3 ± 0.6 c

5823 ± 160 a

6.8 ± 0.0 a

0.80 ± 0.00 a

1.9 ± 0.3

347 ± 74

2.46 ± 0.44

0.44 ± 0.06

  1. Samples from May 2016 were sampled in surface (0–5 cm depth) and samples from May 2017 were sampled at 4 depth (0–2, 2–5, 5–15 and 15–30 cm depth). Mean (n = 3) and standard deviation. Letters indicate significant differences (p < 0.05) among sampling zones of the same site and sampling date (one-way anova followed by a Tukey HSD PostHoc test). GUN: Gunstett site, ETV: Etang-vert site. Mineralization activities and rRNA gene copies are expressed as gram of dry weight soil.