Table 1 Primers used for the generation of CRL5Ozz components.
From: Biophysical and functional study of CRL5Ozz, a muscle specific ubiquitin ligase complex
Gene | Primers | |
|---|---|---|
Ozz | Ozz For | 5′ aaggatccgacgctgctgcctccgag 3′ |
Ozz Rev | 5′ ccgaattctcactcatacttgcagaaatcc 3′ | |
Elongin B | EloB For | 5′ cgggatccatggacgtgttcctcatgatccg 3′ |
EloB Rev | 5′ cggaattctcactgcacggcttgttcattgg 3′ | |
Elongin C | EloC For | 5′ gatcggatccatggatggagaggagaaaacctat 3′ |
Eloc Rev | 5′ gatcggatccaaatttcaactttgattgctatgca 3′ | |
Rbx1 | Rbx1 For | 5′ taactagtgacgcggcagcgatggatgtgg 3′ |
Rbx1 Rev | 5′ cctctagactagtgcccatactttt 3′ | |
Cul5 | Cul5 For | 5′ taggatccatggcgacgtctaatctgttaaa 3′ |
Cul5 Rev | 5′ ccggatccccatgatattcaaaattatgcc 3′ | |
