Table 2 Summary of results of the velocity c(s) analysis of Ozz–EloBC, Cul5–Rbx1 and CRL5Ozz (Ozz–EloBC + Cul5–Rbx1) complex in 10 mM sodium phosphate, 1.8 mM potassium phosphate pH 7.2, 137 mM NaCl and 0.27 mM KCl buffer at 20 °C.

From: Biophysical and functional study of CRL5Ozz, a muscle specific ubiquitin ligase complex

Sample

mg/mla

s20 (Svedberg)b

s20,w (Svedberg)c

M (Da)d

f/f0e

Ozz–EloBC

0.35

3.83 (35%)

3.98

55,325 (57,285)

1.30

5.75 (53%)

5.97

101,738 (114,571)

1.30

Cul5–Rbx1

0.21

4.70 (69%)

4.88

104,744 (103,212)

1.63

CRL5Ozz

0.75

3.52 (8%)

3.66

57,791

1.46

5.09 (6%)

5.28

100,260

1.46

6.93 (55%)

7.20

159,245 (160,497)

1.46

9.02 (19%)

9.38

235,243

1.46

11.96 (5%)

12.42

358,422 (320,992)

1.46

  1. aTotal concentration of sample in mg/ml.
  2. bSedimentation coefficient taken from the ordinate maximum of each peak in the best-fit c(s) distribution at 20 °C with percentage protein amount in parenthesis. Sedimentation coefficient (s-value) is a measure of the size and shape of a protein in a solution with a specific density and viscosity at a specific temperature.
  3. cStandard sedimentation coefficient (s20,w-value) in water at 20 °C.
  4. dMolar mass values (M) taken from the c(s) distribution that was transformed to the c(M) distribution with theoretical values in parenthesis. The Ozz–EloB-C preparation shows monomer and dimer.
  5. eBest-fit weight-average frictional ratio values (f/f0)w taken from the c(s) distribution.
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