Table 3 Best-fit values and estimates of the 2D c(s,f/f0) analyses of Ozz–EloBC and Cul5–Rbx1, and CRL5Ozz (Ozz–EloBC + Cul5–Rbx1) in 10 mM sodium phosphate, 1.8 mM potassium phosphate pH 7.2, 137 mM NaCl and 0.27 mM KCl buffer at 20 °C.

From: Biophysical and functional study of CRL5Ozz, a muscle specific ubiquitin ligase complex

Sample

mg/mla

sw (Svedberg)b

s20,w (Svedberg)c

M (Da)d

(f/f0)we

Rsf

Ozz–EloBC

1.00

5.96 (52%)

6.19

108,998

1.31

4.25

Cul5–Rbx1

0.21

4.82 (59%)

5.00

105,821

1.61

5.10

CRL5Ozz

0.75

6.66 (48%)

6.85

165,688

1.61

4.45

8.12 (18%)

8.43

245,076

1.67

 

9.58 (8%)

9.95

327,193

1.72

7.85

3.16 (7%)

3.28

60,144

1.68

 
  1. aTotal concentration of sample in milligram per milli liter.
  2. bWeight-average sedimentation coefficient sw calculated from the 2D c(s,f/f0) model with percentage protein amount of total protein in parenthesis.
  3. cStandard sedimentation coefficient (s20,w-value) in water at 20 °C.
  4. dEstimated molar mass calculated from (sw,f/f0) from the 2D c(s,f/f0) model.
  5. eWeight-average frictional ratio (f/f0)w calculated from the 2D c(s,f/f0) model.
  6. fStokes radius (nm) obtained from contour plot of the c(s,M) distribution.
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