Figure 1
From: Ketone body and FGF21 coordinately regulate fasting-induced oxidative stress response in the heart
Effects of βOHB on FGF21 expression and signaling in cultured neonatal rat cardiac myocytes. FGF21 concentrations in cultured medium (A) and in cell lysates (B) were measured by ELISA after stimulation of cultured neonatal rat cardiac myocytes with βOHB (0–5 mM) for 24 h Data are mean ± SD (n = 3–4). **p < 0.01 vs control (0 mM) analyzed by one-way ANOVA followed by Dunnett correction for multiple comparisons. (C) mRNA levels for FGF21, PPARα and β-klotho were compared between the vehicle (ctrl) and βOHB (0.5 mM)-treated cardiac myocytes (n = 6). Data are mean ± SD (n = 6). **p < 0.01 vs control analyzed by unpaired Student’s t-test. (D) Representative western blots to assess the relative changes of FGF21 and indicated signaling molecules in response to βOHB at indicated concentrations for 24 h. Full-length blots are presented in Supplementary Fig. S1.
