Table 2 List of handling steps per process for 24-well plate and BECA-D for expansion of EBVST.
Handling steps | ||
|---|---|---|
24-well plate | BECA-D | |
Seeding | Seed PBMCs and LCLs | Add media to media chamber Seed PBMCs and LCLs into cell chamber |
Expansion | Collect and pool cells from each well Wash each well Seed cells (T-cells and LCLs) to each well (with or without IL-2) | Collect cells from cell chamber Wash cell chamber Seed cells (T-cells and LCLs) into cell chamber Expand cell chamber Remove 50% of media from media chamber Add fresh media (with or without IL-2) to media chamber |
Medium change | Remove 50% of media from each well Add fresh media (with or without IL-2) to each well | Add IL-2 to media chamber |
Harvest | Collect and pool cells from each well Wash each well Count cells | Collect cells from cell chamber Wash cell chamber Count cells |
