Table 3 Comparison of assay sensitivity in paired plasma and whole blood.

From: Diagnostic performance of a 5-plex malaria immunoassay in regions co-endemic for Plasmodium falciparum, P. vivax, P. knowlesi, P. malariae and P. ovale

Analyte

PCR-confirmed species

n of paired samples

Plasma

Whole blood

P-value from McNemar’s paired test comparing plasma vs whole blood sensitivity

Threshold (pg/mL)

Sensitivity (95%CI)

Threshold (pg/mL)

Sensitivity (95%CI)

Uncorrected

Yates correction

HRP2

Pf

63

40.84

92.1 (82.4–97.4)

4.31

100 (94.3–100)

0.025

0.044

Pf-LDH

Pf

63

17.81

88.9 (78.4–95.4)

12.64

100 (94.3–100)

0.008

0.014

Pf-LDH

Pm

24

17.81

95.8 (78.9–99.9)

12.64

100 (85.8–100)

0.32

0.62

Pv-LDH

Pv

65

9.14

76.9 (64.8–86.5)

75.66

93.9 (85.0–98.3)

0.0009

0.002

Pv-LDH

Pk

66

9.14

97.0 (89.5–99.6)

75.66

93.9 (85.2–98.3)

0.41

0.54

Pan-LDH

All malaria

220

27.56

87.3 (82.1–91.4)

84.42

95.5 (91.8–97.8)

0.0001

0.0002

CRP

All malaria

220

26,212a

89.6 (84.7–93.3)

13,768.a

80.0 (74.1–85.1)

0.0006

0.0008

  1. PCR polymerase chain reaction, CI confidence interval, Pf Plasmodium falciparum, Pv Plasmodium vivax, Pk Plasmodium knowlesi, Pm Plasmodium malariae, Po Plasmodium ovale, HRP2 Histidine-rich protein-2, LDH lactate dehydrogenase, CRP C-reactive protein.
  2. aUnits of ng/mL.
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