Figure 1 | Scientific Reports

Figure 1

From: Biophysical studies of cancer cells’ traverse-vessel behaviors under different pressures revealed cells’ motion state transition

Figure 1

The design principle of the microfluidic device and time-lapse microscopy images of two typical cells with different traverse-vessel behaviors. (A) Schematic diagram of the double-layer microfluidic chip with CO2 layer (blue) and cell cultivation layer (red) to maintain cell culture environment and culture cells. (B) A photograph of the microfluidic chip with CO2 layer (upper, blue) and cell cultivation layer (bottom, red). Scale bar: 1 cm. (C) Detailed schematic diagrams of the cell cultivation layer with Inlet, Filter, Trap Unit and Outlet. (D) Schematic illustration of experimental design in the research. (E,F) Time-lapse microscopy images and the protrusion length as a function of time for two typical cells under applied pressure difference \(\Delta {\textit{P}}\), 200 mbar and 100 mbar respectively. Scale bar: 20 μm.

Back to article page