Figure 1

CK19 mRNA copy numbers by OSNA assay (n = 214). The cut-off value for micrometastasis (+) of the standard sample was set at 250–5000 copies/μl; over 5000 copies/μl was diagnosed macrometastasis (+ +) and fewer than 250 copies/μl was diagnosed as negative31. Three open circles show false-positive cases and two open squares show false-negative cases.