Figure 4 | Scientific Reports

Figure 4

From: In-vitro NET-osis induced by COVID-19 sera is associated to severe clinical course in not vaccinated patients and immune-dysregulation in breakthrough infection

Figure 4

Dysregulation of myeloid cells in peripheral blood of COVID-19 patients. Application of T-distributed stochastic neighbor embedding (t-SNE). Clusters were named based on the cluster-specific antigen expression patterns in healthy subjects (A) and COVID-19 patients (B). Example of gating strategy based on CD14 and CD16 expression in circulating monocytes to identify non-classical, intermediate and classical monocytes in healthy and COVID-19 patients (C). Detection of percentage and absolute counts of monocyte-like myeloid derived suppressor cells identified as CD14+-HLA-DR- monocytes (D). Color code: grey, healthy subjects; green, mild COVID-19; orange, moderate COVID-10, red, severe COVID-19. COVID-19 severity was defined according to qCSI, based on respiratory rate, pulse oximetry and oxygen flow rate. Stars denote significance according to ANOVA test among the three COVID groups (mild, moderate, severe), *p < 0.005, **p < 0.001.

Back to article page