Figure 3
Versican expression remains similar to wildtype during degeneration. (a)–(c), Immunolabelling for Versican (red) was detected in all the layers of the retina in wildtype, Aipl1-/- and Pde6brd1/rd1 with relatively strong signals in the INL and IPL, particularly in mid and advanced stage Pde6brd1/rd1 and advanced-stage Rho-/- retinae. (d)–(f) Vcan mRNA levels were largely unchanged in Aipl1-/- and Pde6brd1/rd1 retinae (with the exception of a reduction at 6 weeks age of Pde6brd1/rd1 mice). (f) In Rho-/-, Vcan mRNA expression remained similar between P10 and 3 months of age, decreasing slightly, but remained significantly higher than age-matched wildtype mice. N.B. mRNA levels shown for wildtype are the same as in Fig. 1c. Scale bar, 100 µm. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA test with Bonferroni’s correction, black; unpaired t-test for age-matched comparisons between wildtype and disease model, blue; two-way ANOVA test applied for assessments of change over time, red). ONL—outer nuclear layer; OPL—outer plexiform layer; INL—inner nuclear layer; IPL—inner plexiform layer; GCL—ganglion cell layer. Nuclei are counter stained with Dapi (blue).
