Table 1 Details of all the antibodies and conditions for immunofluorescence staining.
Primary antibody | Dilution | Supplier's reference | Incubation time | Secondary antibody | Dilution | Supplier's reference | Incubation time |
|---|---|---|---|---|---|---|---|
Ki67 (rabbit monoclonal) | 1:500 | Ab16667, Abcam, Paris, France | overnight (4 °C) | ALEXA FLUOR 488-conjugated anti-rabbit | 1:200 | Ab150077, Abcam, Paris, France | 60 min (RT) |
Cleaved caspase3 (rabbit polyclonal) | 1:200 | Ab49822, Abcam, Paris, France | 90 min (RT) | ALEXA FLUOR 488-conjugated anti-rabbit | 1:200 | Ab150077, Abcam, Paris, France | 60 min (RT) |
γH2A.X (mouse monoclonal) | 1:200 | JBW301 clone, Merck, Darmstadt, Germany | 60 min (RT) | ALEXA FLUOR 488-conjugated anti-mouse | 1:200 | Ab150113, Abcam, Paris, France | 60 min (RT) |
co-immunostaining of γH2A.X (mouse monoclonal) and DDX4 (rabbit polyclonal) | 1:200 1:800 | JBW301 clone, Merck, Darmstadt, Germany Ab13840, Abcam, Paris, France | overnight (4 °C) | biotinylated goat anti-mouse (+ ALEXA FLUOR 594-conjugated streptavidin, ab272189, Abcam) ALEXA FLUOR 488-conjugated anti-rabbit | 1:200 1:200 | Ab64255, Abcam, Paris, France Ab150077, Abcam, Paris, France | 60 min (RT) |
