Figure 3
From: Altered epithelial barrier functions in the colon of patients with spina bifida
(A) A first macroscopic image of the whole biopsy was first obtained using a wide-field camera in order to screen for the regions of interest within the biopsy. Static FF-OCT images of the region of interest were then obtained at 2 depths. (B) Two images for each subject were assessed. (C) Image of biopsy of Hv stained with Sirius red. (D) Image of biopsy of SB stained with Sirius red. (E) The percentage of collagen per area were significantly lower in patients with SB (p = 0.0003). (F) The percentage of collagen per area after staining with red sirius was significantly lower in patients with SB (p = 0.0075). (G) The mRNA expression of TGF beta was significantly higher in patients with SB compared with Hvs (p = 0.0176). (H) The mRNA expression of MMP2 was significantly higher in patients with SB compared with Hvs (p = 0.0121) and tended to be correlated with the paracellular permeability (p = 0.0516) (I). (J) The TIMP1/MMP1 balance was significantly lower in patients with Spina Bifida (p = 0.0348).
