Figure 1

Transcriptome analysis and evaluation of the Hspa5p. (a) Rank of gene transcription abundance (RPKM). Three cell cultures for transcriptome analysis were performed with the following cell and medium combinations: culture 1: clone 1/G13 medium, culture 2: clone 1/CD DA1 medium, and culture 3: clone 2/G13 medium. Gene transcription was ranked by that on day 4 of culture 1, and the top 20 most highly expressed genes are described. (b) Gene transcription abundance (RPKM) of the three cultures on days 4, 7, 9, 11, and 14. (c) Luciferase assay of high-expression candidate promoters in transient expression cell pools (n = 2). (d) Culture time course of luciferase expression levels of stably expressing cell pools. *P < 0.05 by t-test on day 12 (n = 2). (e) Culture time course of gene expression of IgG (heavy chain). Relative transcription level was calculated using the gapdh gene as a control. *P < 0.05 by t-test on day 12 (n = 2). (f) Culture time course of IgG production per cell (SPR). *P < 0.05 by t-test on day 14 (n = 2). (g) Culture time course of mAb yield (IgG titer). *P < 0.05 by t-test on day 14 (n = 2).