Figure 2
From: Development of a stable antibody production system utilizing an Hspa5 promoter in CHO cells
In silico analysis and characterization of Hspa5p sequence. (a) In silico analysis of promoter sequence of Hspa5p (~ 0.6 kb). Conservation of Hspa5p among Chinese hamster, human, mouse, and rat is shown. Core promoter elements (TATA-box, CpG island, CCAAT-box, and GC-box), cAMP-responsive elements (CREB), and ER stress-responsive elements (ERSE) are described. Transcription start site was identified by mRNA sequence from NCBI database (NM_001246739.2). (b,c) Culture time course of stable pools on days 5, 7, 10, and 13 of SPR (b) and IgG titer (c) with different species of heterogeneous Hspa5p (n = 2). (d,e) Culture time course of stable pools on days 5, 7, 10, and 13 of SPR (d) and IgG titer (e) with different lengths of Hspa5p. *P < 0.05 by t-test on day 13 (n = 2).
