Figure 3
From: Development of a stable antibody production system utilizing an Hspa5 promoter in CHO cells
Evaluation of Hspa5p for different cell lines and IgG subclasses. (a) Culture time course of stable pools on days 4, 7, 10, and 14 of SPR and IgG titer with different IgG subclasses. As a promoter, hEF1αp and Hspa5p (~ 0.6 kb) were used. Regarding IgG subclasses, IgG1, IgG2, and IgG4Pro were used. *P < 0.05 by t-test on day 10 for SPR and on day 14 for IgG titer (n = 3). (b,c) Transcription of IgG (heavy chain) gene (b) and endogenous hspa5 gene (c) on culture day 7. Relative transcription level was calculated using actβ gene as a control.
