Figure 2
From: An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation
Proliferation analysis of CFSE-labeled T lymphocytes after PHA stimulus. T lymphocytes were exposed to 0, 1, and 2 Gy of 220 kV X-rays and cell proliferation was examined at 0, 24, 48, 72, and 96 h. (a) Representative example of CFSE fluorescence profiles of live non-irradiated T lymphocytes. The open black histograms show stimulated CFSE-labeled cells. The solid grey histograms show unstimulated CFSE-labeled control samples. The open grey histograms show the autofluorescence of stimulated control samples, not labeled with CFSE. (b) The precursor frequency (PF) of CFSE-labeled cells for each irradiation dose, at various time points. Individual datapoints of 6 independent experiments are shown, with the mean ± standard deviation. No statistically significant differences were found (p > 0.05) between irradiation doses. (c) Representative example of CFSE fluorescence profiles of live irradiated T lymphocytes visualized as described for (a).
