Figure 3
From: An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation
EdU pulse-chasing of T lymphocytes in whole blood cultures allows for the assessment of the cell cycle progression. T lymphocyte proliferation was stimulated with PHA for 96 h before EdU pulse-labeling. (a) Bivariate distributions of non-irradiated EdU pulse-labeled T lymphocytes showing DNA content (x-axis) and EdU incorporation (y-axis). The green population shows the EdU-positive T lymphocytes. The red population shows the EdU-negative cells. The black arrows indicate the progression of the cells through the cell cycle, over time. The bivariate profiles of one donor are displayed here. Other donors show similar distributions. (b) Bivariate profiles of T lymphocytes exposed to 2 Gy of X-rays, visualized as described for (a). (c) Bivariate profiles of T lymphocytes exposed to 4 Gy of X-rays, visualized as described for (a).
