Table 1 Effect of culture medium and embryokine treatment of embryos on pregnancy outcome at day 30 of gestation.

From: Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium

Medium

Embryokine

Pregnant, fraction and percenta

Odds ratiob

Odds ratio for embryokine within each mediumb

Odds ratio for medium within vehicleb

Odds ratio for medium within CSF2b

Odds ratio for medium within DKK1b

No serum

Vehicle

45/81 (55.6%)

1

1

1

  

No serum

CSF2

35/94 (37.2%)

0.475 (0.256 0.881)

0.472 (0.252 0.887)

 

1

 

No serum

DKK1

35/93 (37.6%)

0.470 (0.253 0.873)

0.468 (0.249 0.879)

  

1

Knockout serum

replacement

Vehicle

58/165 (35.2%)

0.450 (0.259 0.781)

1

0.442 (0.252 0.776)

  

Knockout serum replacement

CSF2

67/182 (36.8%)

0.469 (0.271 0.810)

1.160 (0.738 1.823)

 

1.097 (0.637 1.890)

 

Knockout serum replacement

DKK1

73/201 (36.3%)

0.482 (0.283 0.821)

1.073 (0.691 1.667)

  

1.010 (0.594 1.718)

Fetal bovine serum

Vehicle

54/138 (39.1%)

0.524 (0.298 0.924)

1

0.505 (0.284 0.896)

  

Fetal bovine serum

CSF2

96/196 (49.0%)

0.758 (0.447 1.286)

1.460 (0.925 2.305)

 

1.582 (0.937 2.671)

 

Fetal bovine serum

DKK1

116/286 (40.6%)

0.599 (0.360 0.994)

1.108 (0.714 1.720)

  

1.238 (0.743 2.061)

  1. Abbreviations are as follow: BSA bovine serum albumin, CSF2 colony stimulating factor 2, DKK1 dickopf WNT signaling pathway inhibitor-1.
  2. aPregnancy rate was affected by the interaction between medium and embryokine (P = 0.042) and by the contrast comparing serum replacement (SR) vs fetal bovine serum (FBS) (P = 0.056) and the interaction between effects of BSA vs SR + FBS and CSF2 (P = 0.004) and DKK1 (P = 0.015).
  3. bOdds ratios were calculated after adjustment for farm, sire and the interaction of sire with treatment.
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