Table 2 Effect of culture medium and embryokine treatment of embryos on pregnancy outcome at day 30 of gestation and calving for one farm in which data at calving were recorded.

Medium	Embryokine	Pregnant at day 30, fraction and percent^a	Calving rate, fraction and percent^b	Pregnancy loss, day 30 to calving, fraction and percent^c
No serum	Vehicle	10/18 (55.6%)	8/18 (44.4%)	2/10 (20.0%)
No serum	CSF2	4/13 (30.8%)	2/13 (15.4%)	2/4 (50.0%)
No serum	DKK1	5/16 (31.3%)	5/16 (31.3%)	0/5 (0.0%)
Knockout serum replacer	Vehicle	8/25 (32.0%)	2/25 (8.0%)	6/8 (75.0%)
Knockout serum replacer	CSF2	17/41 (41.5%)	7/41 (17.1%)	10/17 (58.8%)
Knockout serum replacer	DKK1	9/25 (36.0%)	7/25 (28.0%)	2/9 (22.2%)
Fetal bovine serum	Vehicle	13/40 (32.5%)	7/40 (17.5%)	6/13 (46.2%)
Fetal bovine serum	CSF2	17/39 (43.6%)	14/39 (35.9%)	3/17 (17.7%)
Fetal bovine serum	DKK1	26/49 (53.1%)	20/49 (40.8%)	6/26 (23.1%)

Abbreviations are as follow: BSA bovine serum albumin, CSF2 colony stimulating factor 2, DKK1 dickopf WNT signaling pathway inhibitor- 1.
^aThere was an interaction between no serum vs (SR + FBS) and vehicle vs DKK1 (P = 0.084).
^bCalving rate was affected by medium (P = 0.079). Analysis of contrasts indicated that serum replacement (SR) differed from fetal bovine serum (FBS) (P = 0.027), and there were interactions between no serum vs (SR + FBS) and vehicle vs CSF2 (P = 0.050) and no serum vs (SR + FBS) and vehicle vs DKK1 (P = 0.051).
^cThere were no effects of medium, embryokine or the interaction (P ≥ 0.10).

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