Figure 4

In vivo monitoring of two-color (808 nm / 915 nm) PA traces in control and malaria-infected mice with P. yoelii GFP⁺ parasites. (a) Time-resolved detection of PA signal waveform in a control mouse from an ear vessel as compared to skin. (b) In vivo PA traces from a healthy (control) mouse. (c) In vivo PA traces from an uninfected mouse with RBC aggregates. In vivo PA traces from an infected mouse at 13 (d), 16 (e), and 21 (f) days after infection. (g) In vivo verification of laser diode-based Cytophone data by testing the same infected mouse using PAFFC platform integrating PAFC (bottom trace) and fluorescent flow cytometry (FFC) module (top trace). (h) In vivo dependence of the PA rates (number of PA signals per minute) on time after infection.