Figure 5

Ex vivo verification of in vivo Cytotophone data using the blood vessel phantom. Two-color PA trace from (a) control (uninfected mouse) and (b) GFP + P. yoelii-infected mouse blood at 13 days post-infection using Cytophone with two laser diodes at 808 nm and 915 nm. (c) Fluorescent and PA traces from control blood using PAFFC platform (integrating PAFC and FFC modules) with pulse lasers at 671 nm and continuous-wave lasers at 488 nm for generation of PA and fluorescent (FL) signals. (d) Ex vivo fluorescent and PA trace from GFP⁺ P. yoelii-infected mouse blood at day 13 using PAFFC platform. Two-color PA traces were obtained with a two-color Cytophone platform using lasers at 671 nm and 820 nm from (e) control human blood and (f) P. falciparum-iRBCs from in vitro culture. (g) Fragment of trace, demonstrating the temporal coincidence of PA peaks at different wavelengths from the same iRBC.