Figure 7
From: Evaluation of protein production in rice seedlings under dark conditions
Effect of seed size on the protein amount produced in rice seedlings. (a) Representative picture of Nipponbare uncut and cut seeds. (b–e) Protein amount of Nipponbare seedlings germinated from uncut and cut seeds in darkness with water (b) and MS medium and 30 g/l sucrose (c), or in light with water (d) and MS medium and 30 g/l sucrose (e). Relative weight (n = 40) or protein amount (n = 5) is shown. The value of uncut seeds was set as 1. Left two bars show seed weight, and right two bars show the protein amount. **Significant difference by Student's t test (P < 0.01). ns: not significant (P > 0.05). (f) Representative picture of Kasalath, Nipponbare and Kusayutaka seeds. (g) Weight of Kasalath, Nipponbare and Kusayutaka seeds. The three cultivars showed significant difference by ANOVA at P < 0.05. (h) Total protein amount in Kasalath, Nipponbare and Kusayutaka seedlings. Seeds were germinated in light or in darkness with or without supplementation of MS medium and 30 g/l sucrose. Total soluble protein of each seedling (n = 5) was extracted at 11 DAG. No significant difference was observed between the three cultivars except between Kasalath and the other two cultivars in the condition with supplementation of MS medium and 30 g/l sucrose in darkness by ANOVA at P < 0.05.
