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Figure 1

From: Apical-out airway organoids as a platform for studying viral infections and screening for antiviral drugs

Figure 1

Generation of airway organoids with apical-out orientation from hBEC aggregates. (A) Workflow for the generation of apical-out organoids showing the time requirement for each step. (B) Representative brightfield image of aggregates generated by hBECs after 1 to 6 days of incubation in a microwell. Arrow points at a representative aggregate located in the centre of the microwell (scale bar 300 μm). (C) Representative brightfield image of aggregates after initiation of suspension culture (scale bar 200 μm). (D) Representative brightfield image of terminally differentiated Ap-O AO at day 15. Cilia can be detected at the edge of the organoid (scale bar 100 μm).

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