Figure 3
Ap-O AO are susceptible to infection with EV-D68. (A) Representative brightfield images showing the cytopathologic effect of EV-D68 infection over 48 h. Infected Ap-O AO organoids disintegrate to single cells over the course of the infection (scale bars = 300 μm). (B) Viral load of EV-D68 detected by qPCR in the supernatant. Points represent mean values of all biological replicates. Error bars represent the SD (n = 3). (C) Viral titer detected in the sampled medium at 0 to 48 h post infection. Titers are expressed as TCID50. Points represent mean values of all biological replicates. Error bars represent the SD (n = 3). (D) Distribution of AC. TUB (red) and the bound viral particles with VP1 (green) in Ap-O AO 0 hpi with EV-D68. DAPI (blue) visualises the nuclei. Arrows indicate bound viral particles (scale bar = 50 μm). (E) Apical-out airway organoid 6 hpi with EV-D68. The nuclei are visualized with DAPI (blue), VP1 (green) and ds-RNA (red) indicate active viral replication. Arrows point at infected cells with ongoing replication (scale bars = 50 μm).
