Table 2 Sequences of primers and probes used to detect viral RNA load.

From: Apical-out airway organoids as a platform for studying viral infections and screening for antiviral drugs

Target

Primer/probe

Sequence 5′ to 3′

Label

RV-A16

Forward

AGS CTG CGT GGC KGC C

 

Reverse

ACA CGG ACA CCC AAA GTA GT

 

Probe

TCC TCC GGC CCC TGA ATG YGG CTA AYC

6FAM-BBQ

IBV

Forward

TCG CTG TTT GGA GAC ACA AT

 

Reverse

TTC TTT CCC ACC GAA CCA

 

Probe

AGA AGA TGG AGA AGG CAA AGC AGA ACT

6FAM-BBQ

IAV

Forward

GAC AAG ACC AAT CCT GTC ACY TCT G

 

Reverse

AAG CGT CTA CGC TGC AGT CC

 

P610

TTC ACG CTC ACC GTG CCC AGT GAG C

6FAM-BBQ

EV-D68

Forward

TGT TCC CAC GGT TGA AAA CAA

 

Reverse

TGT CTA GCG TCT CAT GGT TTT CAC

 

Probe1

ACC GCT ATA GTA CTT CG

6FAM-BBQ

Probe2

TCC GCT ATA GTA CTT CG

6FAM-BBQ