Figure 1

Experimental design. Breast cancer patients (BCP) and healthy donors (HD) were included in the study. From the patient group, biopsies and tumors were taken before and after NAT, respectively, and IHC and TCR sequencing were performed. Additionally, a section of the tumor sample was processed by enzymatic and mechanical digestion and used to evaluate different cell populations by flow cytometry. A blood sample was also taken from each patient before and after NAT to assess the activation status of T cells by flow cytometry and for TCR sequencing. From the HD group, breast tissue was taken during surgery for IHC, TCR sequencing and flow cytometry. This figure was created using BioRender (https://biorender.com/).