Figure 3

T cell composition in tumor and normal breast tissue. (A) Frequency of CD45⁺ cells from healthy donors (HD) and breast cancer patients (BCP). (B) Frequency of CD3⁺ cells from HD and BCP. (C) Frequency of CD3⁺ cells according to the molecular subtype of breast cancer: luminal A (LA), luminal B (LB), Her2 and triple negative (TN). (D) Percentage of CD4⁺ and CD8⁺ T cells. Distribution of memory subpopulations of CD4⁺ (E) and CD8⁺ T cells (F) evaluated using the CD45RA, CD62L, CCR7 and CD95 markers to define naïve (T_N), stem cell memory (T_SCM), central memory (T_CM), tissue-resident memory-like (T_RM), effector memory (T_EM) and terminally differentiated effector (T_TE) T cells. Pie charts of the distribution of CD4⁺ (G) and CD8⁺ T cell (H) memory subpopulations according to the molecular subtype of breast cancer. (I) Frequency of CD4⁺CD25⁺Foxp3⁺ regulatory T cells (Tregs). (J) Ratio of CD8⁺/Treg cells. (K) Frequency of CD4⁺ and CD8⁺ T cells from HD and patients with positive lymph nodes (PLNs) or negative lymph nodes (NLNs). (L) Treg frequency according to the lymph node state. (M) Frequency of follicular T cells from healthy donors (HD) and breast cancer patients (BCP). Data are represented as the mean ± SEM in (A, B, C, D, E, F, I, and J). Data are represented as violin plots, and lines indicate quartiles in K and J. The p values were calculated using a Mann–Whitney test. *p < 0.05, **p < 0.01, ***p < 0.001.