Figure 5
From: The breast cancer immune microenvironment is modified by neoadjuvant chemotherapy
Response of PBMCs to in vitro stimulation of patients before and after NAT. (A) t-SNE plot for PBMCs with FlowSOM-based clusters. (B) Heat map indicating the expression level of different markers used in flow cytometry for each of the populations determined by FlowSOM grouped by their hierarchical proximity. (C) Spanning tree of the subpopulations determined by FlowSOM with the expression level of each marker within the circle (the size of the circle is proportional to the number of events). (D) Frequency of each FlowSOM subpopulation in the four groups of patient samples. (E) CITRUS-based expression tree for each of the markers in each subpopulation. (F) Histograms of expression of the clusters that differed statistically between the groups of samples. (G) Relative abundance (Log10) of the clusters that significantly differentiate the groups of patient samples. *p < 0.05, **p < 0.01.
