Figure 2

Selective binding of native TDP-43 constructs to Hsp70 isoforms. Microscale thermophoresis was used to measure the binding of ranging concentrations of Hsp70 SBDs to 50 nM of labelled TDP-43_1–102 (A) or TDP-43_102–269 (C). HspA1A and HspA5 were able to bind TDP-43_102–269. No signal was detected for HspA8 binding to TDP-43_102–269. (B) Table of affinity constants extracted from the MST values for TDP-43_1–102. (D) Table of affinity constants extracted from the MST values for TDP-43_102–269. Statistical difference was assessed between HspA1A and HspA5 binding (Welch’s test). Data is presented as Mean ± SD (n = 3). Statistical difference was assessed between HspA1A, HspA5 and HspA8 binding (Brown-Forsythe and Welch ANOVA test).