Figure 2 | Scientific Reports

Figure 2

From: AAV2-mediated gene therapy for Bietti crystalline dystrophy provides functional CYP4V2 in multiple relevant cell models

Figure 2

CYP4V2 expression following AAV2 transduction of cultured cells. (A and B) CYP4V2 expression in HEK293 and ARPE19 cells transduced with AAV2.wtCYP4V2 and AAV2.coCYP4V2, respectively, at multiplicities of infection (MOI) from 10E3 to 10E5, assessed by Western blot. (C and D) CYP4V2 expression in HEK293 and ARPE19 cells transduced with AAV2.wtCYP4V2 and AAV2.coCYP4V2, respectively at MOI of 10E5, assessed by immunocytochemistry. (E and F) CYP4V2 enzyme activity was quantified by Multi-CYP assay in HEK293 and ARPE19 cells transduced with AAV2.wtCYP4V2, AAV2.coCYP4V2 and control AAV2.eGFP. Mean ± SEM (n = 6). **p < 0.01. ****p < 0.00001. ns, not significant.

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