Figure 2 | Scientific Reports

Figure 2

From: Integrated analysis of an in vivo model of intra-nasal exposure to instilled air pollutants reveals cell-type specific responses in the placenta

Figure 2

Deconvolution of Bulk RNA-Seq data. (a) Cell-type deconvolution of bulk RNAseq data based on pseudobulk scRNAseq as a reference. Numbers present a fraction of the total count. Stars represent an FDR-corrected significant difference (t-test) between the detected cell type fractions in AP (air pollution; n = 4 sequenced samples obtained from 6 pregnant mice) and CON (control; n = 4 sequenced samples obtained from 6 pregnant mice) at a level below 0.05. (b) Overlap between the 50 top marker genes for each tissue (Supplementary Table 2) type in the scRNAseq dataset and genes up- or downregulated in AP versus CON detected by bulk RNAseq (Supplementary Table 3). A higher value (depicted with darker color) represents a higher level of overlap. (c) Differential abundance of cell types based on z-scores calculated using cell specific marker genes from scRNAseq analysis which were significantly expressed among all samples and filtered by p value < 0.05. Students t-test was performed to calculate the significance between two groups InvSpT1 = Invasive Spongiotrophoblasts Type 1. InvSpT2 = Invasive Spongiotrophoblasts Type 2.

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