Figure 3

Ultrastructural changes in L. (L) amazonensis promastigotes treated with amphotericin B, FrDCL, SF5-6, SF8 and SF9 obtained from Aspidosperma nitidum bark. (A) Negative control; (B) Solvent control; (C, D and E) amphotericin B: 0.1953 µg/mL, 0.09765 µg/mL and 0.048825 µg/mL, respectively; (F, G and H) FrDCL: 50 µg/mL, 25 µg/mL and 12.5 µg/mL, respectively; (I and J) SF5-6: 50 µg/mL and 25 µg/mL, respectively; (K, L and M) dihydrocorynantheol: 25 µg/mL, 12.5 µg/mL and 6.25 µg/mL, respectively; (N, O and P) SF9 50 µg/mL, 25 µg/mL and 12.5 µg/mL, respectively. The arrowhead indicates a parasite with a short flagellum, a black arrow indicates protuberances in the flagellum and/or a double flagellum, the red arrow indicates a change in cell shape and the asterisk, septation of the cell body. Bars: 10 µm.