Figure 7
Itga2 is downregulated in the SMC-lineage cells differentiating from Myh11∆K/∆K iPSCs. (A) Phase-contrast images of WT and Myh11∆K/∆K iPSCs at day 5 of differentiation by retinoic acid (scale bar = 200 µm). (B) RT-qPCR analysis of Myh11 mRNA isolated iPSCs at days 0 and 3 of treatment with retinoic acid. Gene expression levels were normalised to18s rRNA (n = 4, day 0: p = 0.9714; day3: p > 0.9999). Mann–Whitney U test. RA = retinoic acid. (C) RT-qPCR analysis of Itga2 mRNA isolated iPSCs at day 3 of differentiation by retinoic acid. Gene expression levels were normalised to18s rRNA. *p < 0.05, Mann–Whitney U test.
