Figure 3
From: Stress-induced despair behavior develops independently of the Ahr-RORγt axis in CD4 + cells
Depletion of Rorc does not Induce Spontaneous Behavioral Changes in Male Mice. (A) Loss of gene expression of both Il17 and Rorc in in vitro skewed Th17s from Rorc KO mice and littermate controls by qPCR (n = 3–4/group). T tests (il17: p = 0.0450, Rorc: p = 0.0281). (B) ELISA representing loss of IL-17 in in vitro skewed Th17s from Rorc KO animals (n = 3–4/group). T test (p = < 0.0001). (C) Baseline marble burying (n = 9 or 16/group), (D) sucrose preference (n = 9/group) and forced swim and tail suspension (n = 22 or 37/group), (E) social preference (n = 9 or 19/group), (F) nestlet shredding (n = 22 or 28/group), elevated plus maze (n = 24 or 31/group) and open field (n = 22 or 31/group), and (G) novel object recognition (n = 9 or 19/group) tests. T tests used in D and F. Two-way ANOVA used in E (Cre Neg: p = 0.0010, Cre Pos: p < 0.0001) and G (Cre Neg: p = 0.0014, Cre Pos: p = 0.0002). (H) Kullback–Leibler divergence heatmap and (I) PCA plot of DeepLabCut analyzed behaviors between groups (n = 22 or 32/group). Combined N = 2, male mice.
