Figure 1

(A) Incubation test of RU 486 treated groups. (B) Plasma membrane integrity of RU 486 treated groups. (C) Concentration of cholesterol in post-thaw sperm. (D1) Western blot of CatSper 2 proteins. (D2) The optical intensity of CatSper proteins normalized to β-tubulin. Values with different letters (a–d) differ significantly (P < 0.05), n = 20.