Figure 1

Comparison of the characteristics of ADSCs culture in SFM and FBS containing media. Calculated (a) population doubling time and (b) accumulated cell number. ADSCs cultured in serum free media (CellCor), and FBS containing media (DMEM with 10% FBS) were compared. SFM showed the lower PDT across the whole passage than FBS. At P15, SFM exhibited a higher increase in ACN than FBS. (c) Flow cytometric analysis of the expression surface markers of cultured ADSCs. All ADSCs showed positive expression of CD73, CD90, and CD105 and negative expression of CD14, CD34, CD45, and HLA-DR in both medium except that CD34 in FBS showed partial positive expression. A representative image from four independent experiments is shown. (d) Expression of surface markers by qRT-PCR analysis of total RNAs isolated from ADSCs. (e) Multilineage differentiation potential of ADSCs. All ADSCs were induced toward differentiation into adipocytes (verified by Oil Red O), chondrocytes (verified by Alcian Blue), and osteocytes (verified by Alizarin Red S). A representative image is shown. (f) qRT-PCR analysis of the mRNA expression levels of FABP4 (adipogenic marker), ACAN (chondrogenic marker), and OCN (osteogenic marker). Data represent the mean ± SEM * vs. corresponding passage FBS containing media. * p < 0.05, *** p < 0.001. ADSC adipose-derived stem cell, SFM serum free media, FBS fetal bovine serum, PDT population doubling time, ACN accumulated cell number, FABP4 fatty acid-binding protein 4, ACAN aggrecan, OCN osteocalcin.