Figure 2

Comparison of cellular characteristics after expansion between ADSCs culture in SFM and FBS containing media. (a) Cellular senescence of ADSCs cultured in SFM or FBS. ADSCs at P5 and P10 were seeded and cultured for 24 h, then stained for senescence associated β-galactosidase activity. Red arrows indicate β-galactosidase positive cell; (b) β-galactosidase-positive cells were counted and presented as a percentage. (c) Genetic stability of ADSCs by cytokinesis-block micronucleus assay for MN, NBUDs, and NPBs at P5 and P10. (d) ADSCs cultured in FBS showed a significant increase in the frequency on MN and NBUDs formation at P5, and MN, NBUDs, and NPBs at P10 compared to ADSCs cultured in SFM. (e) Flow cytometric analysis of immune modulation function by co-culture of ADSCs and fluorescently labeled T cells. (f) Fluorescence intensity per cell after co-culture reflected the functional ability of ADSCs to suppress T cell proliferation. T cell proliferation was inhibited more by ADSCs cultured in SFM than ADSCs cultured in FBS. Data are represented as the mean ± SEM. * vs. corresponding passage FBS containing media. * p < 0.05. ADSC adipose derived stem cell, SFM serum free media, FBS fetal bovine serum, MN micronuclei, NBUD nuclear bud, NPB nucleoplasmic bridge.