Figure 3

Comparison of mRNA expression profile. The levels of mRNA expression of ADSCs cultured in SFM and FBS containing media were compared by RNA sequencing. (a) mRNA expression levels in ADSCs from four donors grown in the different media, showed up-regulation of 120 genes in ADSCs cultured in SFM and 201 genes in ADSCs cultured in FBS. (b) Gene ontology terms enriched by differentially expressed genes after cell expansion in the two different media. ADSCs cultured in FBS showed up-regulation of genes involved in aging, apoptosis, cell death, extracellular matrix, immune response, and inflammatory response. Gene expression profile validated by qRT-PCR analysis after cell expansion in the FBS containing media and three different SFMs included (c), aging (d), apoptosis (e), immune response (f), inflammatory response (g), DNA repair (h), and RNA splicing. Data are represented as the mean ± SEM. ** p < 0.01, *** p < 0.001. ADSC adipose-derived stem cell, SFM serum free media, FBS fetal bovine serum, IGFBP5 insulin-like growth factor binding protein 5, SOD2 superoxide dismutase 2, CXCL12 C-X-C motif chemokine ligand 12, PTX3 pentraxin-3, TNFRSF11B tumor necrosis factor receptor superfamily member 11B, CSF1 colony-stimulating factor 1, HMGA2 high mobility group A2 protein, SNRPF small nuclear ribonucleoprotein polypeptide F, SNRPD1 small nuclear ribonucleoprotein D1, SFM, SFM B, and SFM C are CellCor, StemPro, and MesenCult, respectively.