Figure 4

Comparison of protein expression profile. Protein identification and abundance were detected by LC–MS/MS. (a) Protein expression in ADSCs from four donors grown in the two different media, showed up-regulation of 252 proteins in ADSCs cultured in SFM and 356 proteins in ADSCs cultured in FBS. (b) Up-regulated proteins in group FBS were involved in apoptosis, cardiovascular disease, skeletal and muscular disorders, inflammatory response, immune cell trafficking, and cell-mediated immune response. Functional classification analysis validated by western blot after cell expansion in the FBS containing media and three different SFMs included (c) apoptosis, (d) inflammatory response, (e) cardiovascular disease, (f) skeletal and muscular disorders, (g) mRNA stabilization. Data are represented as the mean ± SEM. ADSC adipose-derived stem cell, SFM serum free media, FBS fetal bovine serum, STAT1 signal transducer and activator of transcription 1, PDLIM5 PDZ and LIM domain 5, MYOF Myoferlin, SNRNP70 small nuclear ribonucleoprotein U1 subunit 70, SFM, SFM B, and SFM C are CellCor, StemPro, and MesenCult, respectively.