Figure 1

NF90 family and NF45 is expressed in pancreatic β cells. (A) Expression levels of NF90 family and NF45 were examined in mice tissues by western blot. Gapdh and α Tubulin was used as an internal control. Experiment is representative of n = 3 mice tissues. (B) Western blot analysis of levels of NF90 family and NF45 in pancreas or isolated islet from wild-type mice. Insulin and Gapdh was used as an islet marker and an internal control, respectively. Intensities of specific bands was measured by densitometry. Gapdh was used for normalization of data. Experiment is representative of n = 3 mice pancreas and n = 6 mice islets. (C) Immunofluorescence staining of NF90 family (red, panels I, II, V and VI) and NF45 (red, panels III, IV, VII and VIII) in pancreas section of wild-type mice. Insulin (Green) and DAPI (Blue) was shown as β cells and nuclei, respectively. Bar, 50 µm. In panels I to IV, an islet was shown as a dash-line. Higher magnifications of #1 to #4 in panels I to IV were presented in panels V to VIII. In panels V to VIII, a nucleus was shown as a dash-line.