Figure 3

βNF45−/− mice develops Type 2 diabetes symptoms. (A) Western blot analysis of the protein level of NF90 and NF45 in islets isolated from control and βNF45−/− mice. α Tubulin was used as an internal control and for normalization of data. Experiment is representative of n = 4 mice islets. (B) Immunofluorescence staining of NF90 (red), NF45 (red), Insulin (green) and DAPI (blue) in pancreas section of control and βNF45−/− mice. Bar, 50 µm. An islet was shown as a dash-line. (C) Growth curve of control mice (n = 6) and βNF45−/− (n = 5) mice fed with either ND or HFD at 6–28 weeks of age. Data are expressed as means ± standard deviations and are representative of two independent experiments. (D) Measurement of the amount of blood glucose of ND-feeding control (n = 4) and βNF45−/− mice (n = 6) and HFD-feeding control (n = 13) and βNF45−/− mice (n = 10) at 28 weeks of age. Data were expressed as means ± standard deviations. *P < 0.05 relative to control by a two-tailed Student’s t test. The data included the result from two independent experiments. (E) Measurement of plasma insulin level of ND-feeding control (n = 11) and βNF45−/− mice (n = 16) and HFD-feeding control (n = 13) and βNF45−/− mice (n = 10). Data were expressed as means ± standard deviations. *P < 0.05 relative to control by a two-tailed Student’s t test. The data included the result from two independent experiments. (F) H & E staining of pancreas section of either ND-feeding or HFD-feeding control and βNF45−/− mice at 28 weeks of age. Bar, 1 mm. (G) Islets were manually traced, and the areas were measured as islet area. More than 400 islets from each group were analysed. The total islet areas were divided by the total number of islets examined and the values were presented as dot plots. **P < 0.01 relative to control by a two-tailed Student’s t test. (H) islet size distribution analysis of ND-feeding control (n = 5) and βNF45−/− mice (n = 3) and HFD-feeding control (n = 3) and βNF45−/− mice (n = 3) at 28 weeks of age. The values were presented as dot plots. **P < 0.01 relative to control by a two-tailed Student’s t test. (I) Immunofluorescence staining of P-Histone H3 (red) and DAPI (blue) and bright-field image in pancreas section of control (HFD) and βNF45−/− mice (HFD). Bar, 100 µm. An islet was shown as a dash-line. (J) Signal intensity of P-Histone H3 in islets of control (n = 4) and βNF45−/− mice (n = 4). The intensities were normalized by the number of DAPI in the islet. The values were presented as box plots. *P < 0.05 relative to control by a two-tailed Student’s t test.