Figure 4

Pathway analysis in β cell lines depleted of NF90 and NF45. (A) Schema of an experimental procedure for microarray analysis. Total RNA was prepared from BTC6 cells treated with siNF90 and siNF45 for 7 days. (B) Volcano plots with ‘log2 Fold Change’ on the x-axis and ‘− log10 p-value’ on the y-axis reveal significant changes of genes that exhibited significantly different expression levels between siNTC-treated cells and siNF90–NF45-treated cells (values of p < 0.005, fold change of NF90–NF45-treated cells is > 1.5 and < − 1.5, respectively). > 1.5-fold-change, p < 0.005 and < − 1.5-fold-change, p < 0.005 were indicated as red and blue colours, respectively. (C) Wikipathway analysis using genes pathed the filter criteria mentioned above (> 1.5 or < − 1.5-fold change, P < 0.005) shown in (A). Top 10 pathways were shown as list in order of significance. An arrow indicates top-1 rank by this analysis. (D) qRT-PCR of 5-upragurated genes (Cdkn1a, Pidd1, Zmat3, Casp3 and Ccng3) in p53 signaling shown in (C) in siNTC- and siNF90–NF45-treated cells. The levels of NF90 and NF45 expression were also measured by qRT-PCR. HPRT was used as an internal control. Data are expressed as mean ± standard deviations. (n = 3 per group). *P < 0.05, **P < 0.01 relative to the siNTC-treated cells by a two-tailed Student’s t test.