Figure 5

Analysis of p53 signaling in β cell lines depleted of NF90 and NF45. PG13-luc (wt p53 binding sites) and MG15-luc (mut p53 binding sites) were transfected into BTC6 cells treated with either siNTC or siNF90–NF45. Luciferase activities were determined 72 h after transfection. The firefly luciferase activity was normalized to the results of the renilla luciferase activity. Data are expressed as the mean ± standard deviations. (n = 6 per group). *P < 0.05 relative to the siNTC-treated cells by a two-tailed Student’s t test.