Figure 6 | Scientific Reports

Figure 6

From: NF90–NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway

Figure 6

Dominant negative form of p53 (p53DD) recovers the growth retardation by the knockdown of NF90–NF45 in β cell lines. (A) BTC6 cells were co-transfected with the indicated siRNA with or without p53DD expression vector. Expression level of NF90, NF45, Gapdh, p53 and p53DD were detected by western blot. Gapdh was used as an internal control. (B) Proliferation of BTC6 cells co-transfected with indicated siRNA with or without p53DD expression vector were analyzed by MTS assay. Data were expressed as mean ± standard deviations. (n = 3 per group). *P < 0.05 relative to either siNTC or siNF90–NF45 and p53DD-treated cells by a two-tailed Student’s t test.

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