Figure 5
SjSS BMDMs produce increased inflammatory cytokines in response to TLR stimuli. In Fig. 4 BMDMs from B6 (n = 3 mice) and SjSS (n = 3 mice) mice were treated with TLR7a (imiquimod) and TLR9a (CpG) to stimulate inflammation, AC, or AC plus TLR7i (ODN 20958), or AC plus TLR9i (ODN 4084-F) to inhibit TLR7 and 9 driven inflammation. Cell culture supernatant was collected after a three hour incubation following conclusion of treatment and wash. (A–D) Cell culture supernatant was evaluated for cytokines with a Bio-Plex assay as performed in Fig. 3. The statistical significance was calculated by two-tailed unpaired t-tests where error bars indicate SEM **p < 0.01, and ****p < 0.0001.
