Figure 1

Correlation between transcriptome-based quantification methods and IHC or FCM. The vertical axis shows arbitrary scores obtained by the indicated computational method for RNA-Seq. (a) The horizontal axis shows the densities of immune cells detected by the indicated antibodies (/mm2). (b) The horizontal axis shows the percentages of the indicated antibody-positive cells in the sample as assessed by FCM. The Pearson's correlation coefficient (r) between the two is shown in the upper part of each scatter plot. Transcriptome-based cell quantification methods for inter-sample comparison were validated with IHC (a) and intra-sample comparison with FCM (b).