Figure 2
A blocking odorant inhibits stressor activation of BNSTa excitatory neurons upstream of CRHNs. (a,b) PRVB177, which has Cre-dependent expression of HA-TK (a), was used to infect Cre-expressing CRHNs in CRH-ires-Cre mice (b). (c–e) On d3pi of CRHNs with PRVB177, animals were exposed to restraint or TMT in the presence of 2PE or water. Brain sections were next costained for HA (PRV) and nFos mRNA. The percentage of PRV+ neurons in a given area that were nFos+ was then determined (nFos+ &PRV+/PRV+ (%)). 2PE significantly decreased the percentage of nFos labeled PRV+ neurons in BNSTa in response to restraint and TMT (c). However, 2PE did not significantly affect the percentage of nFos labeled PRV+ neurons in ARC with restraint (d) or those in LPGi with TMT (e). Column heights indicate means, error bars indicate S.E.M., and dots in the same column indicate different animals. n = 4–7 per condition. Unpaired t test, **P < 0.01. (f,g) A higher percentage of BNSTa PRV+ neurons were colabeled for Vglut1/2 than Gad1/2 (f). A large majority of BNSTa PRV+ neurons activated by restraint or TMT were colabeled for Vglut1/2 but not Gad1/2 (g). Column heights indicate means, error bars indicate S.E.M., and dots in the same column indicate different animals. n = 4 per condition. Unpaired t test, **P < 0.01.
