Figure 1 | Scientific Reports

Figure 1

From: The new preparation method for paraffin-embedded samples applying scanning electron microscopy revealed characteristic features in asthma-induced mice

Figure 1

SEM images of Grocott-stained paraffin specimens of bronchial companion artery (Ar) resected from wild-type mice without any treatment. (a)–(h) indicates the same area. (a), (b) PM, 5kv, coating thickness 2.5 nm and 5.0 nm. (c), (d) EM, 5kv, coating 2.5 nm and 5.0 nm. (e), (f) EM, 10kv, coating 2.5 nm and 5.0 nm. (g), (h) EM, 15kv, coating 2.5 nm and 5.0 nm. (a, b) For PM, 5kv, 5.0 nm, image was uniform and difficult to observe the microstructure, while 2.5 nm provided contrast. (a, c) The same 5kv, 2.5 nm coating provided images with higher contrast in EM than in PM. (b, d) In both PM and EM, the 5kv, 5.0 nm coating provided images with higher contrast. In both PM and EM, 5kv, 5.0 nm coating did not depict microstructure. (e, f) EM, 10kv, gave clear images, but 2.5 nm gave better contrast. (g, h) EM, 15kv showed image disturbance due to charge-up (arrows). All scale bars show 25 μm length.

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